United States Department Of Agriculture Research Articles

Scaling use of the rust fungus Puccinia punctiformis for biological control of Canada thistle (Cirsium arvense (L.) Scop.): First report on a U.S. Statewide effort

Canada thistle (Cirsium arvense (L.) Scop., CT) is one of the worst weeds threatening temperate regions of the world. A host-specific rust fungus, Puccinia punctiformis (F. Strauss) Rohl., is known to cause systemic disease of CT, ultimately killing individuals and reducing stand densities. In 2013, it was demonstrated that fall inoculation of rosettes with coarsely ground leaves bearing P. punctiformis telia can successfully initiate epiphytotics. In the same year, a cooperative project between the Colorado Department of Agriculture and United States Department of Agriculture was initiated, in which CT patches across the state of Colorado (USA) were inoculated and tracked over subsequent years for changes in stem density. Here, we report our findings from 8 years (2014–2021) of monitoring effort. At most sites (N = 87), CT stem densities declined, from a mean (±SE) of 87.9 (±6.5) stems to 44.7 (±4.2). These declines however were spatially-autocorrelated, and likely attributable to local growing conditions, as mean annual daily maximum temperature and standard deviation of elevation, as well as climatic conditions around the times of both treatment and monitoring, were found to be important predictors of CT decline. Further, we observed that the amount of inoculum deployed, timing since last release, and method in which it was spread locally at a site were also associated with the magnitude of CT stem decline. These results are indicative of the value of P. punctiformis as a CT biological control agent.

Choline in pregnant women: a systematic review and meta-analysis.

Choline is a critical nutrient. Inadequate choline intake during pregnancy increases the risk of adverse maternal and offspring health. A systematic review and meta-analysis were conducted to examine the current recommendations for choline intake by pregnant women, estimate the overall prevalence of pregnant women with adequate choline intake, and explore associations between maternal choline level and adverse pregnancy outcomes (APOs). Choline recommendations for pregnant women were assessed from eight nutrient guidelines of the United States, United Kingdom, Canada, Australia, Asia, International Federation of Gynecology and Obstetrics, and World Health Organization. Data on the prevalence of pregnant women with adequate choline intake and the association between maternal choline level and APOs were collected from 5 databases up to May 2023. Meta-analyses with random effects and subgroup analyses were performed for the pooled estimate of prevalence and association. Five recent nutrition guidelines from the United States (United States Department of Agriculture), United States (Food and Drug Administration), Canada, Australia, and the International Federation of Gynecology and Obstetrics have emphasized the importance of adequate choline intake for pregnant women. Of 27 publications, 19 articles explored the prevalence and 8 articles explored the association. Meta-analysis of 12 prevalence studies revealed a concerning 11.24% (95% confidence interval, 6.34-17.26) prevalence of pregnant women with adequate choline intake recommendations. A meta-analysis of 6 studies indicated a significant association between high maternal choline levels and a reduced risk of developing APOs, with an odds ratio of 0.51 (95% confidence interval, 0.40-0.65). The existing guidelines highlight the importance of choline in supporting maternal health and fetal development during pregnancy. Furthermore, a high maternal choline level was likely to be associated with a lower risk of APOs. However, 88.76% of pregnant women do not achieve the optimal choline intake. Therefore, specific policies and actions may be necessary to improve choline intake in pregnant women's care and support the well-being of pregnant women. PROSPERO registration no. CDR42023410561.

Assessment of dietary-lifestyle patterns and adherence to the USDA recommendations in Lebanese pregnant women amid the economic crisis: Findings from a national representative cross-sectional study.

As Lebanon's economic crisis become uncontrollable, Lebanese pregnant women face malnutrition, with many having to skip meals and switch to resort to cheap and unhealthy alternatives altogether. The objectives of the study were to assess the dietary and lifestyle patterns of Lebanese pregnant women and to evaluate their diets compliance with the United States Department of Agriculture (USDA) pregnancy recommendations, before and during the Lebanese escalating economic crisis. A cross-sectional study was conducted between April 2021 and January 2022. A validated self-administrated questionnaire was administered during the first, second and third trimesters of pregnancy among 363 women in all Lebanese governorates. Most of the pregnant women were free of diseases. While the majority did not smoke, 14.1% smoked hookah / shisha during pregnancy. The adherence to the USDA recommendations in our sample did not significantly vary prior to and throughout the socioeconomic crisis, and it was generally low. Only the mean consumption of vegetables increased during the socioeconomic crisis (p<0.05). Regarding physical activity, while the proportion of active women slightly decreased during the socioeconomic crisis, around 55% were still active. In conclusion, higher attention should be given to the dietary habits and health of this critical population, through effective interventions that increase awareness and achieve measurable improvements.

Dietary Flavonoid Intakes in France Are Linked to Brewed Tea Consumption and to Socioeconomic Status: Analyses of the Third French Individual and National Food Consumption (INCA3) Survey for Children and Adults

Flavonoids from green and black tea may benefit cardiovascular health. Brewed tea consumption and flavonoid intake in France have not been previously explored. This study assessed the dietary intake of flavonoids among French children and adults, using 3 days’ dietary recall for 3896 persons aged &gt;4 y in the Third French Individual and National Food Consumption Survey (INCA3). Foods consumed by INCA 3 participants were manually matched with the flavonoid content of foods from the French PhenolExplorer database and the US Department of Agriculture expanded flavonoid database (2018 version). The six subclasses of flavonoids were flavan-3-ols, flavanones, anthocyanidins, flavonols, flavones, and isoflavones. Flavonoid intake was stratified by age subgroups (children and adults separately) and examined using socio-demographics and tea consumption patterns. Mean flavonoid intake was 210 mg/d. Flavonoids in the French diet were predominantly flavan-3-ols (147 mg/d), of which tea is the main source. The effects of age, education, income, and socio-professional category (SPC) on flavonoid intake were all significant (p &lt; 0.0001). Brewed tea consumers were 31.88% of French adults and 3.79% of children. Brewed tea consumption and flavonoid intake were highly correlated. The highest brewed tea and flavonoid intakes were found among individuals with the highest SPC and education levels. Flavonoid intake in France was associated with brewed tea consumption and with higher education and income.

Understanding landowner participation in the Conservation Reserve Program in the U.S. High Plains region

Understanding the array of financial and non-financial decision-making drivers of private landowners with vulnerable cropland for enrolling in the United States Department of Agriculture’s Conservation Reserve Program (CRP) is critical for the success and cost-effectiveness of agricultural policies that aim to create long-term agri-environmental security. Following the underpinnings of the Theory of Planned Behavior, we used Structural Equation Modeling to test the predictive validity and impacts of socio-cognitive variables: attitude, subjective norm, and perceived behavioral control, on landowners’ intent to enroll in CRP. We analyzed mailed survey data of landowners in the western High Plains of the United States, where irrigated agriculture is a major economic activity, and cropland consists of vulnerable wetlands that play a role in recharging the Ogallala Aquifer. Overall, landowners had a positive attitude towards the wetland conservation benefits of CRP enrollment and its benefits of preventing soil erosion and sedimentation. In addition, attitudes, subjective norms, and perceived behavior controls positively influenced landowners’ intentions to (re)enroll in CRP. Among the three predictors, subjective norms had the largest influence. Landowners’ understanding of CRP benefits in improving water quality and availability, and opinions from their neighbors and other landowners were the most significant measured variables affecting their intention to enroll. For enhanced outreach and long-term success, we suggest that policymakers recognize and respond to a range of non-financial motivations to better design programs that represent the conservation benefits of wetlands to leverage their willingness to participate. Furthermore, (re)enrollment acres could be enhanced by understanding local barriers related to landowners’ skills and knowledge and fostering collaboration and coordination among different federal conservation programs in the region.

Consumer Behavioural Patterns Towards Milk and Dairy Products: An Analytical Investigation of an Indian Dairy Supply Chain

Background: India has the most significant milk production and consumption level in the world. Fluid milk consumption will rise to 90 million metric tons in FY 2024 (Annual Report 202-23, United States Department of Agriculture). Milk is an essential component of daily diet in every age segment in India. Consumers’ behaviour and consumption patterns are based on their choices and preferences about product quality, price and preferences. This article critically investigates the consumer perception towards indigenous dairy markets and their consumption and behavioural pattern. Methods: Quantitative approach and Multiple Logistic Linear Regression Analysis (MLLRA) technique was used to test the hypothesis to establish a direct and indirect relationship among endogenous and exogenous variables. The sample was drawn from rural and urban regions of India’s top ten milk-producing states. Result: The research findings suggested a significant relationship between the direct and indirect role of consumers buying behaviour characteristics towards milk and dairy products and their satisfaction level. This research is limited to milk processors, dairy companies and dairy scientists in the field of marketing and dairy research. Dairy Farmers, Processors and Producers are vigilant about the quality of food products, especially milk delivered to their end users and their value-addition in the Milk Supply Chain (MSC) and logistics. In the past three years, during the unprecedented COVID-19, consumers’ consciousness level about health and well-being has been argued.

Comparing "Meaningful Use" of Health Information Technology in Pennsylvania: Electronic Prescription Rates of Metropolitan and Rural Counties.

The Health Information Technology for Economic and Clinical Health Act incentivized the adoption of electronic health records (EHRs). Health systems looked to leverage technology to assist in serving populations in health professional shortage areas. Qualitative research points to EHR usability as a source of health inequities in rural settings, making the challenges of EHR usage a subject of interest. Pennsylvania offers a model for investigating rural health infrastructure with it having the third largest rural population in the United States. This study analyzed the adoption of Electronic Prescribing in the 67 Pennsylvania (PA) counties. Physician adoption and usage data for PA and the United States were compared using a t-test to establish a basis for comparison. PA counties were categorized using the United States Department of Agriculture (USDA)'s Rural-Urban Commuting Areas (RUCAs) system. Surescript use percentages were plotted against the RUCA scores of each PA county to create a polynomial regression model. PA office-based physicians, on average, utilize e-prescription tools at the same rate as the national average with 59% of practices utilizing Surescripts as of 2013. There was no significant correlation between Surescript usage and the rural/urban classification of counties in Pennsylvania (R-squared value of 0.06). Pennsylvania was able to adopt health information technology (HIT) infrastructure at the same rate as the national average. Rural and metropolitan definitions do not correlate to meaningful use of HIT, thus usability of HIT cannot be tied to health outcomes. Future studies looking at specific forms of HIT and their ability to decrease the burden of administrative work for clinicians.

Public trust in regulatory agencies and support for policies on agricultural gene drive

AbstractPublic trust in government agencies plays an important role in the formation of public opinion about public policy issues. However, the association between public trust in regulatory agencies and public support for policy development in emergent biotechnologies such as gene drive is not well understood. The United States Department of Agriculture (USDA), the Food and Drug Administration (FDA), and the Environmental Protection Agency (EPA) are tasked with coordinating and regulating biotechnology. Drawing on past literature, this study examines how public trust in these federal agencies is associated with public opinion on various options for gene drive policy. Using data from a nationally representative public opinion survey (n = 1220) conducted in 2021, our statistical analyses show that respondents who report higher levels of trust in regulatory agencies are more likely to support policy proposals that promote gene drive research and ultimately, may lead to regulatory policies that allow gene drive to be researched and employed to manage agricultural pests, establishing a pathway for scientists, developers, producers, and consumers alike to realize the benefits of this technology.

Genetic impact of external Targhee sires at the U.S. Sheep Experiment Station: a case study of introgression.

Sheep breeders requested that the U.S. Sheep Experiment Station (USSES) to participate in national genetic evaluation through the National Sheep Improvement Program (NSIP). The reasons included the need for (1) a comparison of the productivity of industry and United States Department of Agriculture (USDA) lines, (2) transparency of USDA flocks, (3) genetic ties for NSIP by sampling of industry flocks, and (4) development of premium genetic lines for public release. In response, USSES began to incorporate external sires from NSIP participating flocks into the USSES Targhee flock. Our objective, based on a pedigree analysis, was to test if introgression of external genetics into the flock was achieved. The pedigree included 13,189 animals with mean maximum generations, mean complete generations, and mean equivalent complete generations of 4.2, 1.8, and 2.6, respectively. The mean generation interval was 3.1 yr. The reference population was defined as lambs born from 2021 to 2023 (n = 792). Two additional populations were defined as the current mature ewe flock (n = 123) and the current mature rams (n = 14). The Genetic Conservation Index averaged 7.7 for the full population and 25.7 for the reference population. Overall inbreeding was 0.003 for the full population and 0.006 for the reference population. The rate of inbreeding was 0.0003 per generation. Average relatedness was 0.015 for the full population and 0.018 for the reference population. The effective number of founders, effective number of ancestors, and founder genome equivalents contributing to the reference population were 60, 39, and 19.1, respectively. The ratio of the effective number of founders to the effective number of ancestors was 1.5, indicating the presence of genetic bottlenecks. Measures of effective population size ranged from 102 to 547. Of the 704 offspring produced by external sires, 17 ram lambs and 132 ewe lambs were retained for breeding. The USSES sires produced 299 offspring with 2 ram lambs and 51 ewe lambs retained. Incorporating external sires resulted in a cumulative percentage of genetic variance of 48.8, 49.1, and 44.2 of external genetics for the reference population, current mature ewe flock, and current mature rams, respectively. Stakeholder needs were addressed by introgression of external sires and participation in NSIP, but future selection practices need to be modified to maintain a minimum of 50% USSES core genetics in the flock.

Yield and yield components of some advanced Camelina (Camelina sativa L. CRANTZ) genotypes in Bolu ecological conditions

The negative effects of global warming are increasing worldwide and this increase is expected to continue. The camelina plant, which can be grown in marginal areas, stands out among other oilseed plants because it is drought-resistant and less costly. In the research, seeds belonging to 8 camelina genotypes, which were prominent in terms of oil rate, seed and oil yield, were used in the trial established in Bolu province, out of a total of 52 genotypes obtained from the seed bank of the United States Department of Agriculture, Agricultural Research Service and Germany. The study was conducted according to the Randomized Complete Block Design in Bolu Abant İzzet Baysal University, Faculty of Agriculture, Research and Application Field in the 2021-2022 production season. In the trait, each plot consisted of six rows with a row length of 3 m, a row spacing of 20 cm, and in row of 5 cm, with three replications. According to the research results; plant height 66.33-71.00 cm, number of branches 3.17-5.07 number plant-1, 1000 seed weight 1.10-1.24 g, seed yield 1095.4-1436.6 kg ha-1, oil content 36.63-40.37%, protein content 23.65-27.22%, oil yield 408.3-559.8 kg ha-1 and protein yield 279.0-391.3 kg ha-1. It was found that values between. According to the results obtained from the study, the K52 genotype in terms of seed yield, the K11 genotype in terms of oil rate and the K52 genotype in terms of protein rate came to the fore.

Public assistance, living environments, and food insecurity: A comparative community case study

Food insecurity continues to affect certain seg­ments of the U.S. population at the household and individual levels even when the economy is experi­encing growth. This recognition has led to the design and implementation of food assistance programs, such as the Supplemental Nutrition Assistance Program, targeting food hardship in low-income families. This is in addition to other types of government assistance, such as housing subsidies and public housing, as low-income households and individuals face similar challenges in housing security. Concern over “concentrated poverty” in traditional public housing environment has contributed to a shift toward mixed-income developments, envisaged to improve the living conditions and economic opportunities of public-housing residents. This paper provides a compara­tive assessment of food insecurity in traditional and mixed-income public housing communities. It also examines the effect of nonhousing public assis­tance on food insecurity and the temporal relation­ship between the timing of food hardship and the receipt of assistance. Administering a modified version of the U.S. Department of Agriculture (USDA)’s Household Food Security Module to the majority of residents in the two communities, the researchers found negligible differences in food insecurity between recipients and nonrecipients of government assistance. Nevertheless, government assistance appeared to improve the probability of being food secure as it interacted with living envi­ronments, suggestive of greater beneficial effect in the environment of mixed-income housing. The results show that the number of households experi­encing reduced food intake was lowest in the first two weeks and highest during the fourth week of the month.

Historic corn yield, production, and economic value trends in Kansas

AbstractWorld corn (Zea mays L.) production has tripled since the 1960s. However, without new breakthrough innovations the trend is expected to plateau or decrease in the future. The objective of this study was to quantify Kansas corn production, economic value, productivity, annual production variation, yield gap trends, and in due course, identify future areas for research. Corn variety performance tests and United States Department of Agriculture (USDA) data were used for this study. Results showed that the area planted to corn in Kansas increased from 0.5 million in 1880s to over 2 million ha by 2022, with an increase of 0.05 million ha year−1 between 1984 and 2022. The value of corn produced in Kansas also increased significantly from below $1 billion annually prior to 1990s to the current value of approximately $3–$4 billion. The average corn yield gain from 1972 to 2000 was 132 kg ha−1 year−1, which declined to 12 kg ha−1 year−1 during 2000 to the early 2020s. Also, a relatively high yield variability occurred from 1970 to the early 2020s compared with earlier years (1866–1970), and a 42%–68% gap between the potential and actual corn yields was identified. We concluded that there is a decrease in the rate of corn yield increase and developing farmer awareness and their adoption of new corn production technology, increasing longevity of the Ogallala Aquifer, and integrated research should be priorities to avert the decreasing trend of corn productivity and further increase corn economic value.

Вплив зрошення мінералізованими водами на процеси засолення ґрунтів Південно-Бузької та Кам’янської зрошувальних систем

Вплив зрошення мінералізованими водами на процеси засолення ґрунтів Південно-Бузької та Кам’янської зрошувальних систем

Impacts of Crop Type and Climate Changes on Agricultural Water Dynamics in Northeast China from 2000 to 2020

Northeast China (NEC) is one of the most important national agricultural production bases, and its agricultural water dynamics are essential for food security and sustainable agricultural development. However, the dynamics of long-term annual crop-specific agricultural water and its crop type and climate impacts remain largely unknown, compromising water-saving practices and water-efficiency agricultural management in this vital area. Thus, this study used multi-source data of the crop type, climate factors, and the digital elevation model (DEM), and multiple digital agriculture technologies of remote sensing (RS), the geographic information system (GIS), the Soil Conservation Service of the United States Department of Agriculture (USDA-SCS) model, the Food and Agriculture Organization of the United Nations Penman–Monteith (FAO P-M) model, and the water supply–demand index (M) to map the annual spatiotemporal distribution of effective precipitation (Pe), crop water requirement (ETc), irrigation water requirement (IWR), and the supply–demand situation in the NEC from 2000 to 2020. The study further analyzed the impacts of the crop type and climate changes on agricultural water dynamics and revealed the reasons and policy implications for their spatiotemporal heterogeneity. The results indicated that the annual average Pe, ETc, IWR, and M increased by 1.56%/a, 0.74%/a, 0.42%/a, and 0.83%/a in the NEC, respectively. Crop-specifically, the annual average Pe increased by 1.15%/a, 2.04%/a, and 2.09%/a, ETc decreased by 0.46%/a, 0.79%/a, and 0.89%/a, IWR decreased by 1.03%/a, 1.32%/a, and 3.42%/a, and M increased by 1.48%/a, 2.67%/a, and 2.87%/a for maize, rice, and soybean, respectively. Although the ETc and IWR for all crops decreased, regional averages still increased due to the expansion of water-intensive maize and rice. The crop type and climate changes jointly influenced agricultural water dynamics. Crop type transfer contributed 39.28% and 41.25% of the total IWR increase, and the remaining 60.72% and 58.75% were caused by cropland expansion in the NEC from 2000 to 2010 and 2010 to 2020, respectively. ETc and IWR increased with increasing temperature and solar radiation, and increasing precipitation led to decreasing IWR in the NEC. The adjustment of crop planting structure and the implementation of water-saving practices need to comprehensively consider the spatiotemporally heterogeneous impacts of crop and climate changes on agricultural water dynamics. The findings of this study can aid RS-GIS-based agricultural water simulations and applications and support the scientific basis for agricultural water management and sustainable agricultural development.

Plant Infrastructure

Abstract This essay analyzes Miami as a place where plants are a major component of urban infrastructure. The centrality of tropical plants to the growth of Miami connects that city to the history of empire, where control of plant matter was the violent model for the standardization and distribution practices of modern infrastructure. In Miami, the US Department of Agriculture established a Plant Introduction Garden in 1898, with “Agricultural Explorer” David Fairchild activating networks connecting India, Kew Gardens, and Washington to bring mostly Asian tropical fruits, shrubs, and flowers to South Florida. A close reading of archives of botanical gardens, plant nurseries, and community organizations shows that Miami plant infrastructure was created jointly by these elite political/scientific networks and by vernacular, informal networks of Black labor migration and horticultural know-how. The essay focuses on the cultivation of Miami’s best-known plant product, the Haden mango, and the vital role in its propagation played by Black Bahamian gardener Nathan Sands, whose letters to his employer David Fairchild are preserved in the latter’s papers. Recovering the stories of people like Nathan Sands is vital to understanding the development of a global metropolis whose plant infrastructure erased the Black horticultural work on which it depended.

A Field Survey of Grapevine Germplasm Susceptible to Eutypa Dieback

Eutypa dieback of grapevine is a trunk disease that impacts vineyard productivity worldwide. Grape germplasm is typically evaluated for resistance to Eutypa dieback through controlled inoculations in the greenhouse, although the high level of replication required of this approach (40 plants per genotype) can limit the total number of genotypes evaluated. An alternative approach is to evaluate naturally infected genotypes in the field. We rated the incidence and severity of vines with the diagnostic leaf symptoms of Eutypa dieback and the incidence of mortality among such vines of 973 Vitis vinifera accessions (planted in duplicate) at the US Department of Agriculture, National Clonal Germplasm Repository in Davis, CA, USA, which is maintained as a living collection for grape research. Across 3 years and spanning a total of 5 years (2011, 2013, and 2015), 120 accessions had leaf symptoms in one or more years (“susceptible accessions”). Courbu blanc [Davis Vitis identification tag (DVIT) 2313], Frankenthal blanc (DVIT 2115), and Pinot gris (DVIT 0907) were the only accessions with leaf symptoms each year. Accessions with the most severe leaf symptoms (a rating of 5 points) were Chasselas Napoleon (DVIT 0375) and Queen of the Vineyard (DVIT 0496). We identified susceptible accessions—namely, those related to ‘Chasselas’ and ‘Muscat’—with a shared genetic background, based on a previous single nucleotide polymorphism genotyping effort of the collection. Especially for grapevine, a long-lived perennial that is meant to produce a crop for decades, knowledge of susceptible accessions and their pedigrees can help inform breeding programs and studies on the host response to infection.

Perspective: Leveraging Electronic Health Record Data Within Food Is Medicine Program Evaluation: Considerations and Potential Paths Forward

Government, health care systems and payers, philanthropic entities, advocacy groups, nonprofit organizations, community groups, and for-profit companies are presently making the case for Food is Medicine (FIM) nutrition programs to become reimbursable within health care services. FIM researchers are working urgently to build evidence for FIM programs’ cost-effectiveness by showing improvements in health outcomes and health care utilization. However, primary collection of this data is costly, difficult to implement, and burdensome to participants. Electronic health records (EHRs) offer a promising alternative to primary data collection because they provide already-collected information from existing clinical care. A few FIM studies have leveraged EHRs to demonstrate positive impacts on biomarkers or health care utilization, but many FIM studies run into insurmountable difficulties in their attempts to use EHRs. The authors of this commentary serve as evaluators and/or technical assistance providers with the United States Department of Agriculture’s Gus Schumacher Nutrition Incentive Program National Training, Technical Assistance, Evaluation, and Information Center. They work closely with over 100 Gus Schumacher Nutrition Incentive Program Produce Prescription FIM projects, which, as of 2023, span 34 US states and territories. In this commentary, we describe recurring challenges related to using EHRs in FIM evaluation, particularly in relation to biomarkers and health care utilization. We also outline potential opportunities and reasonable expectations for what can be learned from EHR data and describe other (non-EHR) data sources to consider for evaluation of long-term health outcomes and health care utilization. Large integrated health systems may be best positioned to use their own data to examine outcomes of interest to the broader field.

Presence of foodborne pathogens and survival of generic Escherichia coli in an organic integrated crop-livestock system

IntroductionIntegrated crop-livestock systems (ICLS) use animals to graze crop residues or cover crops before planting fresh produce and provide ecosystem services to support organic vegetable production. However, there is a risk of foodborne pathogen transfer to fresh produce because grazing may introduce enteric foodborne pathogens into the soil via animal feces, which may subsequently be transferred to the produce.MethodsTo examine the effect of cover crop use and the risk of cover crop grazing on the contamination of soil and produce by foodborne pathogens in ICLS, a three-year (2019–2021) experimental study was conducted in organically managed plots, which were assigned three different treatments (fallow without cover crop or grazing, cover crop without grazing, or cover crop with grazing by sheep) in a maize/tomato rotation. During the three years of the experiment, a total of 184 pre- and post-graze fecal samples and 96 samples of tomatoes were collected to test for foodborne pathogens (Escherichia coli O157, non-O157 Shiga toxin-producing Escherichia coli (STEC), and Listeria (L.) monocytogenes). Soil samples were collected monthly until 126–171 days after grazing (824 in total) to examine the presence of foodborne pathogens, and generic E. coli (MPN/g) was quantified to compare its persistence among the three treatments.Results and DiscussionWe did not detect any foodborne pathogens from harvested tomatoes in 2020 and 2021. One non-O157 STEC positive soil sample (0.1%, 1/824) was detected in the fallow treatment, and one L. monocytogenes-positive (1.1%, 1/92) was detected from the post-graze fecal samples. When assessing proportions of generic E. coli positive and counts of generic E. coli in the soil samples using mixed effect zero-inflated negative binomial models, soil samples collected in the graze cover crop treatment plot showed significant increases in the counts of generic E. coli until 61–82 days post grazing, but no difference was observed after 96–123 days, compared to the baseline of the fallow treatment. Findings from generic E. coli counts support the use of the United States Department of Agriculture (USDA) National Organic Program (NOP) 90- or 120-day interval rule between applying raw manure and harvesting in organic farming into ICLS. Additionally, we confirmed that commercial organic compost application before cover crop seeding in the winter had no significant effect on the proportions and counts of generic E. coli in the soil of the following growing seasons. This longitudinal field trial confirmed that the effect of sheep grazing on foodborne pathogen contamination in ICLS is minimal but further studies comparing the genetic associations between fecal and soil samples would be necessary to distinguish the source of foodborne pathogen contamination.

Almond defect and freshness inspection system using hyperspectral imaging and deep learning techniques

Almonds, recognized as a nutritious and low-calorie snack rich in unsaturated fats and essential nutrients, are primarily imported from California. Despite undergoing grading control by the United States Department of Agriculture (USDA), concerns persist regarding the presence of defective kernels. Manual hand-picking is the conventional method for almond processing; however, prolonged picking can reduce accuracy due to visual fatigue. Consumers prioritize freshness due to fungi’s potential for aflatoxin production, emphasizing the need for vigilant almond consumption. In response to these challenges, this study presents an innovative approach that employs a lightweight VGG16 and an enhanced MobileNet model for identifying defects based on RGB images of almonds. This method effectively classifies broken, scratched, and malformed kernels. Additionally, hyperspectral imaging (HSI) data in the 400–1000 nm range were collected to distinguish insect-damaged from undamaged kernels and assess almond freshness. For comparative analysis, various band selection algorithms were applied to extract distinct spectral bands. For insect damage identification, we propose a merged CNN model created by simultaneously combining 1D- and 2D-CNNs, allowing for the concurrent extraction of spectral and spatial characteristics. This approach achieved a remarkable ACC of 0.9905 and a Kappa value of 0.9521. Meanwhile, in evaluating freshness, PCA was employed to select the first 30 principal components as vital bands. Our proposed lightweight 1D-CNN delivered an ACC of 0.9768 and a Kappa value of 0.9527 in freshness assessment.

Financial toxicity in early-onset colorectal cancer: A National Health Interview Survey study

BackgroundFinancial toxicity is increasingly recognized as a devastating outcome of cancer treatment but is poorly characterized in patients with early-onset colorectal cancer. Young patients are particularly vulnerable to financial toxicity as they are frequently underinsured and may suffer significant disruptions to professional and financial growth. We hypothesized that financial toxicity associated with colorectal cancer treatment confers long-lasting effects on patients’ well-being and disproportionately impacts patients diagnosed at <50 years of age. MethodsA retrospective cross-sectional analysis of the National Health Interview Survey from years 2019 to 2021 was performed. Patients with a history of colorectal cancer were included and stratified by age at diagnosis. Randomly selected age-matched controls with no cancer history were used for comparison. The primary endpoint was financial toxicity, as assessed by a composite score formulated from 12 National Health Interview Survey items. The secondary endpoint was food security assessed by the United States Department of Agriculture’s food security scale, embedded in the National Health Interview Survey. ResultsWhen compared to age-matched controls, patients with colorectal cancer experienced significant financial toxicity, as reflected by a composite financial toxicity score (P = .027). Within patients with colorectal cancer, female sex (adjusted odds ratio = 1.46, P = .046) and early-onset disease (adjusted odds ratio = 2.11, P = .002) were found to significantly increase the risk of financial toxicity. Patients with early-onset colorectal cancer more frequently experienced food insecurity (P = .011), delayed necessary medical care (P = .053), mental health counseling (P = .043), and filling prescriptions (P = .007) due to cost when compared to patients with average-onset colorectal cancer. ConclusionColorectal cancer is associated with significant long-term financial toxicity, which disproportionately impacts patients diagnosed at <50 years of age. Targeted interventions are warranted to reduce financial toxicity for patients with high-risk colorectal cancer.